Figure 14. Isobaric labeling-based multiplexed quantification of gangliosides.

A) Labeling of BODIPY-modified LacCer with iTRAQ™. The BODIPY was functionalized with azide (−N₃) which reacts iTRAQ™ via click chemistry. B) Labeling of intact gangliosides using aminoxyTMT™. Gangliosides were chemoselectively oxidized at the trihydroxy functional group of sialic acid using NaIO₄ and the resulting aldehyde is labeled with aminoxyTMT™. Reprinted with permission from Barrientos, R.C. & Zhang, Q. Anal. Chem. 90, 2578–2586 (2018)[140], © 2018 American Chemical Society.