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Glycoblotting involves the following steps: 1) Glycans with a free reducing end (e.g., aldehyde) are mixed with amine-functionalized beads; 2) Reductive amination takes place to conjugate the glycans on the beads; 3) After washing, the glycans are released by transamination, typically using an aminooxy-functionalized compound, R-ONH2; 4) The released glycans are purified by HILIC-SPE; and 5) Analyzed by LC-MS/MS.
