Figure 1.

Expression of Palmitoyltransferases (PATs) and Protein Palmitoylation in Glioblastoma Multiforme (GBM) Cells

(A) The expression of different DHHCs in GBM is summarized on the basis of immunohistochemical findings of the Human Protein Atlas. (B) Acyl-biotin exchange (ABE) detection of the palmitoylated proteins in normal brain tissue (NBT) and GBM tissues. HAM+, hydroxylamine-treated sample; HAM−, Tris-treated control (no hydroxylamine). (C) Reverse transcription polymerase chain reaction (RT-PCR) analysis of the mRNA levels of the 23 known PATs in six GBM cell lines. β-Actin and GAPDH were used as controls. (D) 2-Bromopalmitate (2BP; 50 μM), cerulenin (Cer; 25 μM), and tunicamycin (Tun; 2.5 μM) inhibited protein palmitoylation in SF126cells. ∗∗∗p < 0.001, unpaired t test.