Figure 2.

Inhibition of Palmitoylation-Repressed Cell Growth and Proliferation

(A) Palmitoylation inhibitors decrease cell survival of SF126 cells. SF126 cells were plated onto 96-well plates and treated with different concentrations of 2BP (50 μM), Cer (25 μM), or Tun (2.5 μM) for 48 h, followed by enumeration of surviving cells. (B) Palmitoylation inhibitors decreased SF126 cell viability. SF126 cells were plated onto 96-well plates and treated with 2BP (50 μM), Cer (25 μM), or Tun (2.5 μM) for 48 h, followed by the evaluation of cell viability using the CCK-8 assay. (C) Palmitoylation inhibitors decrease colony formation of SF126 cells. SF126 cells were plated onto 10-cm plates and treated with 2BP (50 μM), Cer (25 μM), or Tun (2.5 μM) for 2 weeks when the number of cell colonies was determined. Scale bar, 10 μm. (D) SF126 cells were treated with 2BP (50 μM), Cer (25 μM), or Tun (2.5 μM) for 48 h, and cell proliferation was assessed using the Edu assay. Scale bar, 100 μm. ∗∗∗p < 0.001, unpaired t test. ns, not significant.