Figure 3.

Inhibition of Palmitoylation Induces G₂ Cell Cycle Arrest and Cell Death

(A–C) Effects of palmitoylation inhibitors on the cell cycle. (A) SF126 cells were treated with 2BP (50 μM), Cer (25 μM), or Tun (2.5 μM) for 48 h, and then the cell cycle distribution was analyzed via flow cytometry. (B and C) The sub-G₁ (B) and G₂/M (C) cell populations were quantified. (D and E) Effect of palmitoylation inhibitors on apoptosis in SF126 cells. Cells were treated with 2BP (50 μM), Cer (25 μM), or Tun (2.5 μM) for 48 h and analyzed for apoptosis using annexin V-propidium iodide double staining and flow cytometry (D). The apoptotic cell population was quantified (E). ∗p < 0.05, ∗∗∗p < 0.001, unpaired t test. ns, not significant.