Figure 5.

Inhibition of Palmitoylation Triggers Endoplasmic Reticulum (ER) Stress

(A) Localization of four DHHC proteins in SF126 cells. ZDHHC4, ZDHHC8, ZDHHC17, and ZDHHC22 (green) co-localize with the ER marker calnexin (red). Scale bar, 50 μm. (B) SF126 glioblastoma cells were treated with 2BP (50 μM), Cer (25 μM), or Tun (2.5 μM) for 48 h, and the cytoplasmic distribution of PDI (red) was observed via confocal microscopy. Scale bar, 20 μm. (C) Structural changes in the ER in SF126 cells treated with 2BP (50 μM), Cer (25 μM), or Tun (2.5 μM) for 48 h, and observed via transmission electron microscopy. Scale bars, 0.5 μm. n = 20.