Figure 6.

Inhibition of Palmitoylation Activates Inositol-Requiring Enzyme 1α (IRE1α) and Causes Death in SF126 Cells

(A–C) SF126 cells were treated with 2BP (50 μM; A), Cer (25 μM; B), or Tun (2.5 μM; C) for the indicated periods. Whole-cell lysates were harvested and analyzed for phosphorylated and total IRE1α, phosphorylated and total c-Jun N-terminal kinase (JNK), spliced (XBP1s) and unspliced X-box binding protein 1 (XBP1)u, CCAAT-enhancer binding protein homologous protein (CHOP), pro-caspase-3, cleaved caspase-3, and the loading control, β-tubulin. ∗∗∗p < 0.001, unpaired t test.