Table 2.
The producing systems of enterovirus-related virus-like particle (VLP).
| VLP-producing systems | Yield capacity | Properties | Status | Ref. |
|---|---|---|---|---|
| Baculovirus-insect cell | Moderate (64.3 mg/L) | Moderate-yield; Relatively high cost; Large stocks (cell & viruses); Contamination risk of virus | Lab | (Chung et al. 2010) |
| Saccharomyces cerevisiae yeast | Low (0.25 mg/L) | Low-yield; Low cost; Ease in manipulation | Lab | (Li et al. 2013) |
| Pichia pastoris yeast | High (270 mg/L) | High-yield; Low cost; Easy manipulation | Clinical trial (CXSL1900022) | (Yang et al. 2019) |
| Recombinant vesicular stomatitis virus (rVSV) | – |
Attenuated(ΔM51); Replication-competent and may have adverse effects |
Lab | (Yan et al. 2016) |
| Recombinant adenovirus 5 (Ad-EVVLP) | – |
Replication-incompetent(ΔE1/ΔE3); 3C-specific cellular immunity Ad-EVVLPs from EV71 genes can protect against CVA16 infection |
Lab | (Tsou et al. 2015) |