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. 2020 Sep 29;10:15969. doi: 10.1038/s41598-020-72724-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Strain-specific gene copy number variations on chromosome 35 at non-permissive temperatures. Normalised copy numbers for indicated genes (LdBPK_35xxxx) on chromosome 35 is shown for (A, B) HSP23^+/+-HT, (C, D) HSP23^−/− esc1/2-HT, (E, F) enlargement of the amplified region on chromosome 35 between positions 420,000 and 490,000. Sequencing read coverage is shown for HSP23^+/+ (LT, black), HSP23^−/− esc1/2 (HT, blue) and HSP23^−/− cl. 2/3 (LT, grey). Open reading frames and corresponding gene IDs are indicated in grey for non-amplified or blue for amplified CDSs; positions of SIDER2 repeat elements are indicated in red. LT:25 °C, HT:37 °C.