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. 2020 Sep 29;10:15969. doi: 10.1038/s41598-020-72724-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Abundance of CK1.2 in HSP23^−/− esc1/2 mutants. (A) Western blot analysis of CK1.2 in HSP23^+ / + and HSP23^−/− esc1/2 using a rabbit anti-CK1.2 antibody (SY3535) (1:250). A chicken anti-HSP70 (1:5000) antibody was used as a loading control. (B) CK1.2 protein level was quantified using ImageJ Fiji Software (Version 2.0.0) and first normalised to Coomassie loading control and then to the HSP23 ^+ / + (37 °C) sample. Statistical testing was carried out by Kruskal–Wallis test; *p < 0.05, **p < 0.01; (n = 6).