Figure 4.

Subtoxic Aβ_1-42 oligomers increase BACE1 translation. Neuroblastoma cells were treated with subtoxic concentrations of Aβ_1-42 oligomers (0.25 μM) for 24 h. BACE1, p-eIF2α, and eIF2α expressions were studied by (a) western blot and (d) immunofluorescence. The bands corresponding to (b) BACE1/tubulin and (c) p-eIF2α/eIF2α were quantified and expressed as A.U. Data are expressed as arbitrary units (A.U.) and represent mean ± SEM of 4-7 independent experiments. ^∗p < 0.05vs. control cells by Student's t-test. BACE1 expression by immunofluorescence analysis was quantified (e) and expressed as fluorescence intensity (F.I.). Data are mean ± SEM of 3 independent experiments. ^∗∗∗p < 0.001vs. control cells by Student's t-test. Bars represent 5 μm.