Fig. 2. Irradiation increases prostate cancer cell surface expression of MHC Class I and PD-L1.
In vitro FACS analysis of MHC Class I and control transferrin receptor expression following treatment of TRAMP-C1 cells with 6 Gy RT in the presence (a) or absence (b) of 1 ng/mL γIFN. FACS analysis of PD-L1 expression following RT in the presence (c) or absence (d) of concomitant γIFN treatment. Data are presented as relative expression normalised to untreated control and analysed using ordinary two-way ANOVA with Tukey’s post hoc adjustment for multiple comparisons (n = 3 independent experiments). *p < 0.05; **p < 0.01; *** p < 0.001, ****p < 0.0001.