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. 2020 Jul 9;123(7):1089–1100. doi: 10.1038/s41416-020-0956-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — FACS analysis of the tumour immune microenvironment of TRAMP-C1 tumour allografts at the 7-day (a) and tumour regrowth to ≥400 mm³ (b) time-points post initiation of 3 × 5 Gy RT compared to untreated controls, with representative plots shown (c). Pie charts represent the proportion of CD45⁺ leucocytes within the total population of live cells. Quantification of PD-1 expression on gated CD45⁺CD8⁺ T-cells following treatment of TRAMP-C1 allograft tumours with 3 × 5 Gy RT compared to untreated controls (d). Data are presented as mean percentage of total live cells ± SEM and analysed by the unpaired t-test (n = at least 4 independent tumour samples analysed) *p < 0.05.