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. 2020 Sep 29;10:15938. doi: 10.1038/s41598-020-72854-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Analysis of the effect of GlmR on YvcJ by dynamic light scattering (DLS). (A) Volume weighted size distributions of 24 µM YvcJ in the presence of 0.4 mM UDP-GlcNAc and in the absence or in the presence of 7 µM GlmR at 25 °C using DLS. In black YvcJ is alone with the UDP-sugar. In black dashed line GlmR is added to YvcJ with a ratio of 1:0.3 YvcJ:GlmR. Three independent measurements were performed for each sample. (B) Real time correlation of intensity over time of YvcJ and GlmR in the presence of 0.4 mM UDP-GlcNAc average of triplicate. In black YvcJ is alone with the UDP-sugar and in black dashed GlmR is added to YvcJ with a ratio of 1:0.3 YvcJ:GlmR.