Table 2.
List of plasmids and primers used in this study.
| Plasmids | Primers | Sequences 5′–> 3′ | References |
|---|---|---|---|
| pET21-YvcJ | YvcJ 5′ | GTCAGGGGGGGGGATCCATGAGTGTTAGTG | 21 |
| YvcJ 3′ | TTTTGTCCCCTCGAGTTTCCGGCTTCT | ||
| pET21-YvcJ(K22A) | yvcJK22A5' | GGAATGTCGGGAGCGGGGGCAACTGTCGCGATCCAAAGC | 21 |
| yvcJK22A3' | GCTTTGGATCGCGACAGTTGCCCCCGCTCCCGACATTCC | ||
| pQE30-GlmR | GlmR-pQE30-fin | TTCAAGGCTCTGCAGTCATTCTTTCAGT | 29 |
| GlmR-pQE30-deb | AGAAGCCGGAGGATCCATGGGACAAA | ||
| pET21-GlmS | GlmS-deb-Bam | AGGAAGGGATCCATGTGTGGAATCGTAGGTTA | This work |
| GlmS-fin-Xho | GTTAAACTCGAGCTCCACAGTAACACTCTTCG | ||
| pQE30-GNA-1 | 32 | ||
| pT25 and pT25-Zip | 30 | ||
| pT18 and pT18-zip | 30 | ||
| pT25-YvcJ | T25yvcj-5 | CGGGCTGCAGGGAGTGTTAGTGAATCACATG | This work |
| T25yvcj-3 | TACCCGGGGATCCTCTTATTTCCGGCTTCTCTTTTC | ||
| pT18-GlmR | GlmR-T18-deb | CGGGTACCGATGGGACAAAAGCCG | 29 |
| GlmR-T18-fin | CAAGCTTTCTTTCAGTAAATCAAC | ||
| pT25-GlmR | GlmR-T25-deb | GGGCTGCAGGACAAAAGCCGAAAATC | 29 |
| GlmR-T25-fin | GCGGATCCTCATTCTTTCAGTAAATCAAC | ||
| pT18-GlmR (R300A) and pT25-GlmR (R300A) | R300AglmR-5 | ACGTATAAAAATGACGTAATAGCTCACGATACACATAAAGTGGCC | This work |
| R300AglmR-3 | GGCCACTTTATGTGTATCGTGAGCTATTACGTCATTTTTATACGT | ||
| pT18-GlmR (Y264A) and pT25-GlmR (Y264A) | Y264AglmR-5 | CCCGACGAAATAAAACGTAAGGCCGAAATGGAATCGGCGCGTCCT | This work |
| Y264AglmR-3 | AGGACGCGCCGATTCCATTTCGGCCTTACG-TTTTATTTCGTCGGG |