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. 2020 Sep 29;10:15942. doi: 10.1038/s41598-020-72991-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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miR-369-3p, NOS2, IL6 and TNFα expression levels in non-inflamed and inflamed regions of intestine from IBD patients. Total RNA samples isolated from non-inflamed (N) and inflamed (P) regions of intestine were obtained from 11 IBD patients and were analyzed for miR-369-3p (A), NOS2 (B), IL6 (C) and TNFα (D) by qRT-PCR. miR-369-3p in the inflamed areas was significantly lower than those in non-inflamed tissue. Expression data of miR-369-3p were normalized to the endogenous control miR-26a-5p. NOS2, IL6 and TNFα expression levels were normalized to the housekeeping gene Gapdh. The dot-plot graphs represent the mean ± SEM. *p < 0.05; ***p < 0.0001.