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. 2020 Sep 29;11(9):813. doi: 10.1038/s41419-020-03018-3

Fig. 1. Promotive or suppressive function on migration and invasion by TRIM29 overexpression or knockdown in PTC cells, respectively.

Fig. 1

a TRIM29 endogenous expression was investigated using western blot analysis in a panel of PTC cells. b K1 and TPC1 were infected with lentivirus containing empty or TRIM29 construct, TRIM29 expression was confirmed by western blot analysis. c, d Control and TRIM29 overexpression K1 (c) and TPC1 (d) cells were plated on 6-well, and viable cell numbers were counted daily for 3 days. e, f The migration of control or TRIM29 overexpression K1 and TPC1 cells were evaluated by Matrigel-uncoated Transwell. Representative photographs were presented (e) and their numbers were counted (f). g, h The invasion of control or TRIM29 overexpression K1 and TPC1 cells were evaluated by Matrigel-coated. Transwell Representative photographs were presented (g) and their numbers were counted (h). i IHH4 cells were infected with gRNA guided TRIM29 using CRISPR/Cas9 system, western blot was performed using the indicated antibodies. j Control and TRIM29 knockdown IHH4 cells were plated on six-well, and viable cell numbers were counted daily for 3 days. k, l The migration and invasion of control or TRIM29 knockdown IHH4 cells were evaluated by Matrigel-uncoated or Matrigel-coated Transwell, Representative photographs were presented (k) and their numbers were counted (l).The data are presented as the mean (SD) of three repeated experiments. *P < 0.05, n.s. not significant.