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. 2020 Sep 29;11(9):813. doi: 10.1038/s41419-020-03018-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a TRIM29 and FN1 protein levels were investigated using western blot in paired fresh papillary cancer (T) and adjacent noncancerous (N) tissues, and representative images were provided. b Scatter plot showing the correlation between TRIM29 and FN1 in papillary cancer tissues. Pearson’s coefficient tests were performed to assess statistical significance. c FN1 expression was investigated in K1 and TPC1 cells with ectopic TRIM29 overexpression. d FN1 expression was investigated in IHH4 cells with TRIM29 knockdown. e–g FN1 expression of control and TRIM29 overexpression TPC1 cells was knocked down using lentivirus containing shRNAs specific against FN1 (shFN1). FN1 knockdown efficiency was confirmed using western blot (e), invasion of cells were analyzed using Matrigel-coated Transwell, representative photographs were presented (f) and their numbers were counted (g). h–j Control or TRIM29 knockdown IHH4 cells were transfected with empty or FN1 containing eukaryotic expression vectors. FN1 expression was confirmed using western blot analysis (h), invasion of cells were analyzed using Matrigel-coated Transwell, representative photographs were presented (i) and their numbers were counted (j). The data are presented as the mean (SD) of three repeated experiments. *P < 0.05, n.s. not significant.