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. 2020 Sep 29;11(9):813. doi: 10.1038/s41419-020-03018-3

Fig. 5. TRIM29 inhibits premiR-873 cleavage by suppressing it recruiting Dicer and TRBP.

Fig. 5

a, b premiRNA-873 expression levels were analyzed using real-time RT-PCR in control or TRIM29 overexpression K1 and TPC1 cells (a), also in control or TRIM29 knockdown IHH4 cells (b). c, d primiRNA-873 expression levels were analyzed using real-time RT-PCR in control or TRIM29 overexpression K1 and TPC1 cells (c), also in control or TRIM29 knockdown IHH4 cells (d). e, f Dicer and TRBP expression were investigated in K1 and TPC1 cells with ectopic TRIM29 overexpression (e), also in IHH4 cells with TRIM29 knockdown (f). g, h RIP was performed using the antibody against TRBP and Dicer, enrichment of premiR-873 was analyzed using qRT-PCR in TPC1 cells (g), and IHH4 cells (h). i, j Relative expression levels of miR-873-3p was also etected by qRT-PCR in K1 and TPC1 cells (i) by TRIM29 overexpression and in IHH4 cells (j) by TRIM29 knockdown. k Scatter plot also showing the negative co-expression between TRIM29 and has-miR-873-3p in papillary cancer tissues according to online database (starbase.sysu.edu.cn). The data are presented as the mean (SD) of three repeated experiments. *P < 0.05, n.s. not significant.