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. 2020 Sep 29;10:16024. doi: 10.1038/s41598-020-72681-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Treatment of Mdr2KO mice with Ghr and DG reduces apoptosis and necrosis markers in the liver. Frozen sections of livers from male Mdr2KO (A) and FVBN (B) mice treated with vehicle, DG or Ghr, were processed for fluorescence labeling of apoptosis (green) and necrosis (red) markers, while the cell nuclei were stained in blue using DAPI, as described under “Methods”. Panels (A) and (B) present representative images of each treatment group. (C, D) Graphs of quantitative determinations of apoptosis and necrosis, respectively, by image analysis using Image J software. Number of animals in each treatment, 4. Number of images per each sample, 10. p < 0.05, *, DG or Ghr vs vehicle. #, Mdr2KO vs FVBN mice.