Appendix 1—key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Mus musculus) | ARR2-PB-Cre (Pbsn-Cre), C57BL/6 (Mus musculus) | Wu et al., 2001 | RRID:IMSR_JAX:026662 | (Mus musculus; male) |
| Strain, strain background (Mus musculus) | Gata3flox, C57BL/6 (Mus musculus) | Grote et al., 2006 | (Mus musculus; male) | |
| Strain, strain background (Mus musculus) | Gata3GFP, C57BL/6 (Mus musculus) | Grote et al., 2006 | (Mus musculus; male) | |
| Strain, strain background (Mus musculus) | Rosa26GATA3, C57BL/6 (Mus musculus) | Grote et al., 2006 | (Mus musculus; male) | |
| Strain, strain background (Mus musculus) | Gata3bio, C57BL/6 (Mus musculus) | Dr. Busslinger, IMP, Vienna | (Mus musculus; male) | |
| Strain, strain background (Mus musculus) | Rosa26BirA, C57BL/6 (Mus musculus) | Wood et al., 2016 | RRID:IMSR_JAX:010920 | (Mus musculus; male) |
| Strain, strain background (Mus musculus) | Krt5CreERT2, C57BL/6 (Mus musculus) | Van Keymeulen et al., 2011 | RRID:IMSR_JAX:029155 | (Mus musculus; male) |
| Strain, strain background (Mus musculus) | Krt8CreERT2, C57BL/6 (Mus musculus) | Choi et al., 2012 | (Mus musculus; male) | |
| Strain, strain background (Mus musculus) | Rosa26LstopLTdTomato, C57BL/6 (Mus musculus) | Madisen et al., 2010 | RRID:IMSR_JAX:007909 | (Mus musculus; male) |
| Strain, strain background (Mus musculus) | Ptenflox , C57BL/6 (Mus musculus) | Trotman et al., 2003 | (Mus musculus; male) | |
| Strain, strain background (Mus musculus) | Bmp5SE, C57BL/6 (Mus musculus) | Kingsley et al., 1992 | RRID:IMSR_JAX:000056 | (Mus musculus; male) |
| Strain, strain background (Mus musculus) | SCID-beige, C57BL/6 (Mus musculus) | Charles River | (Mus musculus; male) | |
| Cell line (Mus musculus) | CaP2 (Mus musculus) | Jiao et al., 2007 | male prostate | |
| Cell line (Mus musculus) | CaP8 (Mus musculus) | Jiao et al., 2007 | male prostate | |
| Transfected construct (Human) | BMP5_TRC3 LentiORF puromycin V5 (pLX317) (human) | Sigma-Aldrich | TRCN0000472902 | mouse UGSM |
| Transfected construct (Human) | TRC3 LentiORF puromycin V5 (pLX317) empty (human) | Sigma-Aldrich | mouse UGSM | |
| Antibody | CD45 (30-F11; rat monoclonal) | Biolegend | (1/500) | |
| Antibody | TER119 (TER-119; rat monoclonal) | Biolegend | (1/1,000) | |
| Antibody | CD31 (MEC13; rat monoclonal) | Biolegend | (1/1,000) | |
| Antibody | CD49f (GoH3; rat monoclonal) | Biolegend | (1/2,000) | |
| Antibody | EpCAM (G8.8; rat monoclonal) | Biolegend | (1/500) | |
| Antibody | SCA1 (D7; rat monoclonal) | Biolegend | (1/500) | |
| Antibody | TROP2 (goat polyclonal) | R&D | (1/100) | |
| Antibody | KRT5 (chicken polyclonal) | Biolegend | 905901 | (1/500) |
| Antibody | KRT8/18 (guinea pig polyclonal) | Fitzgerald | 20R-CP004 | (1/200) |
| Antibody | GATA3 (H-48; rabbit polyclonal) | SantaCruz | sc-9009 | (1/100) |
| Antibody | Ki67 (SolA15, rat monoclonal) | eBioscience | 14-5698-82 | (1/200) |
| Antibody | pAKT S473 (rabbit monoclonal) | CST | 4060 | (1/500) |
| Antibody | AKT (rabbit polyclonal) | CST | 9272 | (1/500) |
| Antibody | α-TUBULIN (DM1A; mouse monoclonal) | Sigma | T9026 | (1/2000) |
| Antibody | IRDye 800CW Donkey anti-Rabbit IgG | licor | 926-32213 | (1/10,000) |
| Antibody | IRDye 680RD Donkey anti-Mouse IgG | licor | 926-68072 | (1/10,000) |
| Recombinant DNA reagent | MISSION pLKO.1-Puro shRNA scrambled (mouse) | Sigma-Aldrich | ||
| Recombinant DNA reagent | MISSION pLKO.1-Puro shRNA Bmp5 (mouse) | Sigma-Aldrich | TRCN0000065609 | |
| Recombinant DNA reagent | MISSION pLKO.1-Puro shRNA Bmp5 (mouse) | Sigma-Aldrich | TRCN0000065610 | |
| Recombinant DNA reagent | MISSION pLKO.1-Puro shRNA Bmp5 (mouse) | Sigma-Aldrich | TRCN0000065611 | |
| Sequence-based reagent | Bmp5_Fw | Sigma-Aldrich | TTACTTAGGGGTATTGTGGGCT | |
| Sequence-based reagent | Bmp5_Rv | Sigma-Aldrich | CCGTCTCTCATGGTTCCGTAG | |
| Sequence-based reagent | Gata3_Fw | Sigma-Aldrich | GTGGTCACACTCGGATTCCT | |
| Sequence-based reagent | Gata3_Rv | Sigma-Aldrich | GCAAAAAGGAGGGTTTAGGG | |
| Sequence-based reagent | Krt5_Fw | Sigma-Aldrich | GAGATCGCCACCTACAGGAA | |
| Sequence-based reagent | Krt5_Rv | Sigma-Aldrich | TCCTCCGTAGCCAGAAGAGA | |
| Sequence-based reagent | Krt14_Fw | Sigma-Aldrich | CCTCTGGCTCTCAGTCATCC | |
| Sequence-based reagent | Krt14_Rv | Sigma-Aldrich | TGAGCAGCATGTAGCAGCTT | |
| Sequence-based reagent | Krt18_Fw | Sigma-Aldrich | ACTCCGCAAGGTGGTAGATGA | |
| Sequence-based reagent | Krt18_Rv | Sigma-Aldrich | TCCACTTCCACAGTCAATCCA | |
| Sequence-based reagent | Krt8_Fw | Sigma-Aldrich | CAAGGTGGAACTAGAGTCCCG | |
| Sequence-based reagent | Krt8_Rv | Sigma-Aldrich | CTCGTACTGGGCACGAACTTC | |
| Sequence-based reagent | Trp63_Fw | Sigma-Aldrich | AGCAGCAAGTATCGGACAGC | |
| Sequence-based reagent | Trp63_Rv | Sigma-Aldrich | CGTCTCACGACCTCTCACTG | |
| Sequence-based reagent | Ly6a_Fw | Sigma-Aldrich | CCATCAATTACCTGCCCCTA | |
| Sequence-based reagent | Ly6a_Rv | Sigma-Aldrich | GGCAGATGGGTAAGCAAAGA | |
| Sequence-based reagent | Itga6_Fw | Sigma-Aldrich | CGCTGCTGCTCAGAATATCA | |
| Sequence-based reagent | Itga6_Rv | Sigma-Aldrich | AAGAACAGCCAGGAGGATGA | |
| Sequence-based reagent | Epcam_Fw | Sigma-Aldrich | GCTGTCATTGTGGTGGTGTC | |
| Sequence-based reagent | Epcam_Rv | Sigma-Aldrich | CACGGCTAGGCATTAAGCTC | |
| Sequence-based reagent | Ppia_Fw | Sigma-Aldrich | GTGCCAGGGTGGTGACTTTACACG | |
| Sequence-based reagent | Ppia_Rv | Sigma-Aldrich | TCCCAAAGACCACATGCTTGCCA | |
| Sequence-based reagent | Bactin_Fw | Sigma-Aldrich | TTGCTGACAGGATGCAGAAGGAGA | |
| Sequence-based reagent | Bactin_Rv | Sigma-Aldrich | ACTCCTGCTTGCTGATCCACATCT | |
| Sequence-based reagent | Bmp5_prom_Fw-8450 | Sigma-Aldrich | TCGGGTGGACCAGATTTAAG | |
| Sequence-based reagent | Bmp5_prom_Rv-8390 | Sigma-Aldrich | CAGCCATTCACGAAGTTCTCT | |
| Sequence-based reagent | Bmp5_prom_Fw-5942 | Sigma-Aldrich | TGAAAGTGGAGATGGGGAAG | |
| Sequence-based reagent | Bmp5_prom_Rv-5827 | Sigma-Aldrich | CCCAGTTTTGGAGGTTCAGA | |
| Sequence-based reagent | Bmp5_prom_Fw+11268 | Sigma-Aldrich | AAAGGGAAAAGTGCTCACCA | |
| Sequence-based reagent | Bmp5_prom_Rv+11312 | Sigma-Aldrich | TCCTCCCTCAGCTCAAAGAA | |
| Sequence-based reagent | Bmp5_prom_Fw+11859 | Sigma-Aldrich | TTGGAAGAGTTCCGATGAGG | |
| Sequence-based reagent | Bmp5_prom_Rv+11947 | Sigma-Aldrich | CAGAGTGGGTGGCAACTTCT | |
| Sequence-based reagent | Bmp5_prom_Fw+24561 | Sigma-Aldrich | GTGAGGTGGCTCAGCATGTA | |
| Sequence-based reagent | Bmp5_prom_Rv+24607 | Sigma-Aldrich | CCAGGGATGGATCTCAGGT | |
| Sequence-based reagent | Cyp19a1_prom-322_Fw | Sigma-Aldrich | GCAAATGCTGCTGATGAAAT | |
| Sequence-based reagent | Cyp19a1_prom-207_Rv | Sigma-Aldrich | ACCTTATCATCTCGCCCTTG | |
| Sequence-based reagent | Cdh1_prom-175_Fw | Sigma-Aldrich | GAACGACCGTGGAATAGGAA | |
| Sequence-based reagent | Cdh1_prom-98_Rv | Sigma-Aldrich | TCCTCCACCCCTGTCTGTAG | |
| Sequence-based reagent | R26wt_geno_Fw | Sigma-Aldrich | AAGGGAGCTGCAGTGGAGTA | |
| Sequence-based reagent | R26wt_geno_Rv | Sigma-Aldrich | CCGAAAATCTGTGGGAAGTC | |
| Sequence-based reagent | R26Tomato_Fw | Sigma-Aldrich | CCCCGTAATGCAGAAGAAGA | |
| Sequence-based reagent | R26Tomato_Rv | Sigma-Aldrich | GAGGTGATGTCCAGCTTGGT | |
| Sequence-based reagent | Bmp5wt_geno_Fw | Sigma-Aldrich | TAAGGACAAGGGAAACCCTC | |
| Sequence-based reagent | Bmp5SE_geno_Fw | Sigma-Aldrich | TAAGGACAAGGGAAACCCTT | |
| Sequence-based reagent | Bmp5_geno_Rv | Sigma-Aldrich | GAACCATTTCACCAGCTCCT | |
| Sequence-based reagent | Rosa26Gata3-wt_geno_Fw | Sigma-Aldrich | AAAGTCGCTCTGAGTTGTTAT | |
| Sequence-based reagent | Rosa26Gata3_geno_Rv | Sigma-Aldrich | GCGAAGAGTTTGTCCTCAACC | |
| Sequence-based reagent | Rosa26wt_geno_Rv | Sigma-Aldrich | GGAGCGGGAGAAATGGATATG | |
| Sequence-based reagent | Gata3_flox_geno_Fw | Sigma-Aldrich | GTCAGGGCACTAAGGGTTGTT | |
| Sequence-based reagent | Gata3_flox_geno_Rv | Sigma-Aldrich | TGGTAGAGTCCGCAGGCATTG | |
| Sequence-based reagent | Gata3GFP_geno_Fw | Sigma-Aldrich | GGCCTACCCGCTTCCATTGCT | |
| Sequence-based reagent | Gata3GFP_geno_Rv | Sigma-Aldrich | TATCAGCGGTTCATCTACAGC | |
| Sequence-based reagent | Pten_flox-wt_geno_Fw | Sigma-Aldrich | AAAAGTTCCCCTGCTGATGATTTGT | |
| Sequence-based reagent | Pten_flox-wt_geno_Rv | Sigma-Aldrich | TGTTTTTGACCAATTAAAGTAGGCTG | |
| Sequence-based reagent | Cre_geno_Fw | Sigma-Aldrich | AGGTGTAGAGAAGGCACTTAGC | |
| Sequence-based reagent | Cre_geno_Rv | Sigma-Aldrich | CTAATCGCCATCTTCCAGCAGG | |
| Peptide, recombinant protein | EGF | Peprotech | 315-09 | 10 ng/ml |
| Peptide, recombinant protein | hBMP5 | Aviscera Bioscience | 00013-01-100 | 50 ng/ml |
| Peptide, recombinant protein | hNOGGIN | RayBiotech | 230-00704-100 | 180 ng/ml |
| Peptide, recombinant protein | Collagenase/hyaluronidase | Stemcell Technologies | . 07912 | |
| Peptide, recombinant protein | dispase II | Roche | 4942078001 | (5 U/ml) |
| Peptide, recombinant protein | DNase I | Roche | 11284932001 | 0.1 mg/ml |
| Peptide, recombinant protein | matrigel | Corning | CACB354234 | |
| Peptide, recombinant protein | BPE | Life technologies | 13028014 | 25 ug/ml |
| Peptide, recombinant protein | b27 SUPPLEMENT | Life technologies | 17504044 | |
| Peptide, recombinant protein | collagenase A | Bioshop | COL004 | |
| Peptide, recombinant protein | fibronectin | Sigma-Aldrich | 11051407001 | |
| Peptide, recombinant protein | collagen type I | Corning | 354236 | |
| Peptide, recombinant protein | proteinase K | biobasic | PB0451 | |
| Peptide, recombinant protein | RNase A | Roche | 10109169001 | |
| Commercial assay or kit | In Situ Cell Death Detection Kit | Roche | 12156792910 | |
| Commercial assay or kit | P1 Primary Cell 4D-Nucleofector Kit | Bioscience | V4XP-1024 | |
| Commercial assay or kit | RNeasy micro kit | Qiagen | 74004 | |
| Commercial assay or kit | Green-2-go mastermix | BioBasic | QPCR004 | |
| Commercial assay or kit | TruSeq Stranded Total RNA Sample Preparation Kit | Illumina | ||
| Chemical compound, drug | tamoxifen | Toronto Research Chemicals | T006000 | 3 mg |
| Chemical compound, drug | K02288 | AdooQ Bioscience | A14311 | 10 uM |
| Chemical compound, drug | Y-27632 | ApexBio | A3008 | 10 uM |
| Chemical compound, drug | 4-hydroxy-tamoxifen | Toronto Research Chemicals | H954725 | 500 ng/ml |
| Chemical compound, drug | 5α-dihydrotestosterone | steraloids | A2570-000 | 1nM |
| Chemical compound, drug | phosphatase inhibitor cocktails (Phostop) | Sigma | 4906845001 | |
| Chemical compound, drug | Fixable Viability Dye eFluor 780 | ebioscience | 65-0865-18 | |
| Software, algorithm | Prism 6.0 | GraphPad | ||
| Software, algorithm | ImageJ | Fiji | ||
| Software, algorithm | FlowJo | LLC | ||
| Software, algorithm | DIVA | BD Biosciences | ||
| Software, algorithm | L-calc | Stem cell technologies | ||
| Software, algorithm | matrix2png program | Pavlidis and Noble, 2003 |