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. 2020 Sep 29;10:16022. doi: 10.1038/s41598-020-72783-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Schematics and sequences analyzed for the seven genes of interest. (A) Syt3; (B) Lrrtm4; (C) Nrxn1; (D) Nrxn3; (E) Shank1; (F) Dlg4; (G) Grid1. Genes are represented as horizontal lines with rectangles representing exons and grey ovals representing CpG islands. The sequence analyzed by bisulfite pyrosequencing is shown in the insert, with the CG sites in bold and underlined.