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. 2020 Jul 7;123(7):1154–1163. doi: 10.1038/s41416-020-0970-z

Fig. 2. IQGAP1 promotes anoikis resistance, migration and invasion of HCC cells.

Fig. 2

a The qRT-PCR and western blotting analysis of IQGAP1 levels in attached or detached HCC cells treated with or without Matrigel. b The efficiency of stable upregulation of IQGAP1 in HepG2 cells and stable downregulation of IQGAP1 in HepG2.2.15 cells was validated by qRT-PCR and western blotting analysis. c The anchorage-independent growths of indicated HCC cells were determined by soft agar colony formation assays. d The cell death of indicated HCC cells under suspension condition was determined by trypan blue exclusion assay. e Indicated HCC cells were placed on poly-HEMA-coated plates to prevent cell adhesion, and then followed by western blotting analysis with indicated antibodies. f Indicated HCC cells were subjected to transwell migration and invasion assays. The results were expressed as mean ± SD from at least three independent experiments. *P < 0.05, **P < 0.01 and ***P < 0.001.