Fig. 5. IQGAP1 activates Src/FAK signalling pathway.

a Indicated HCC cells maintained in suspension condition were exposed to 20 mM NAC or 10 μM H₂O₂, respectively. After incubation for 48 h, immunoblot analysis was performed with indicated antibodies. b Indicated HCC cells were transfected with EGFP-Rac1T17N or EGFP-Rac1Q61L plasmid, respectively, and then analysed by western blotting with indicated antibodies. c HepG2 and HepG2.2.15 stably expressing IQGAP1 were treated with Y15 or PP2, and then followed by soft agar colony formation assays. d Indicated HCC cells were maintained in suspension condition, treated as in c and then followed by trypan blue assay. e Indicated HCC cells were maintained, treated as in d and analysed by western blotting with indicated antibodies. f Indicated cells treated with Y15 or PP2 were subjected to transwell migration and invasion assays. The results were expressed as mean ± SD from at least three independent experiments. *P < 0.05, **P < 0.01 and ***P < 0.001.