Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Aug 1;31(17):1904–1916. doi: 10.1091/mbc.E19-11-0600

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 Bilodeau et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License.

PMC Copyright notice

FIGURE 5: — Loss of PI4KIIIβ alters cell shape distribution. (A) Two independent lines of PI4KIIIβ-deleted cells have different population shape distributions (depicted in the right panels) in both freely migrating conditions and in the wound of a scratch. The fraction of elongated cells in either line of CRISPR cells is significantly (p < 0.001, t test) lower than in a population of WT cells. Similarly, the fraction of elongated cells in either line of CRISPR cells is significantly (p < 0.001, t test) higher than in a population of WT cells. Results are the mean and SD of triplicate independent measurements of at least 200 cells each from a minimum of 20 randomly selected fields (B) Representative fields of each cell type. Scale bar is 20 μm. (C) Re-expression of either WT- or N162A-PI4KIIIβ restores elongated and multidirectional shape distribution to the CRISPR cells. Expression of KD-PI4KIIIβ does not rescue cell shape changes and their elongated of multidirectional composition is similar to the CRSPR control and significantly different from WT cells (p < 0.05, t test). Results are the mean and SD of quadruplicate independent measurements of at least 200 cells from at least 20 randomly selected fields (D) Distance traveled over time was monitored for 50 WT NIH3T3 cells with either elongated or multidirectional morphologies. Tracks for 10 cells of each type are shown. Multidirectional cells have a significantly reduced average angular rotation that elongated cells (p < 0.05, t test). (E) Quantification of migration patterns in WT cells with either multidirectional or elongated morphologies. Mean angular rotation or velocity is presented as the mean and SD of 50 cells collected over at least 30 min. Angular rotation is significantly lower in the multidirectional population compared with the elongated one (p < 0.05, t test). Comparison between Angular Rotation and Velocity between WT and CRISPR lines. Mean angular rotation or velocity is presented as the mean and SD of 50 cells collected over at least 30 min.