FIGURE 3:

NESs with charged β-strands bind either stronger or weaker to CRM1 E571K. (A) Details of the NES-binding grooves of NES-bound CRM1 from crystal structures of WT (left) and E571K (right) CRM1 bound to the Mek1^NES. CRM1 is in gray, and the NES is in cyan. (B) Details of the NES-binding grooves of eIF4E-T^NES-bound WT CRM1. CRM1 is in gray, and the NES is in green. Electron density from composite omit maps (2F_o-F_c contoured in blue mesh to 1σ) are shown for key residues in A andB. (C) Binding affinities of interactions between wild type and mutated Mek1^NES, eIF4E-T^NES, and Paxillin^NES with WT and E571K CRM1.