FIGURE 5:

Additional NES  β-strand residues contribute interactions with WT and E571K CRM1. (A) Details of the NES-binding grooves of Mek1^NES (green, left) and (B) Mek1NQ^NES (pink) bound to WT CRM1 (gray). (C) Binding affinities of interactions between wild type and mutated Mek1^NES with WT and E571K CRM1. (D, E) Details of the NES-binding grooves of PKI^NES (cyan, D) and PKI^NESDE (pink, E) bound to WT CRM1 (gray). (F) Binding affinities of interactions between wild type PKI^NES and PKI^NESDE with WT and E571K CRM1. Electron density from composite omit maps (2F_o-F_c contoured in blue mesh to 1σ) are shown for  β-strands in A, B, D, and E.