TABLE 1:
Binding affinities (Kd) of a diverse set of NESs for WT and E571K CRM1.
| Binding affinity (Kd) | ||||
|---|---|---|---|---|
| NES | Sequence | CRM1WT | CRM1(E571K) | Kd,E571K/Kd,WT |
| Mek1NES | TNLEALQKKLEELELDEQ | 70 nM[40–130 nM]a | 5 nM[3–8 nM] | 0.071 |
| P73NES | NFEILMKLKESLELMELVP | 2.0 μM[1.7–2.4 μM]a | 1.5 μM[1.0–2.2 μM] | 0.75 |
| hRio2NES | RSFEMTEFNQALEEIKG | 2.4 μM[1.7–4.7 μM]a | 1.9 μM[1.4–2.5 μM] | 0.79 |
| X11L2NES | SSLQELVQQFEALPGDLV | 1.5 μM[1.1–2.0 μM]a | 1.3 μM[0.9–1.8 μM] | 0.87 |
| mDia2NES | SVPEVEALLARLRAL | 1.7 μM[1.0–2.7 μM]a | 1.5 μM[1.0–2.2 μM] | 0.88 |
| IκβαNES | EQMVKELQEIRLEPQ | 22 μM[17–28 μM] | 21 μM[17–27 μM] | 0.95 |
| Snupn1NES | MEELSQALASSFSVSQDLNS | 12 μM[10–15 μM]a | 21 μM[15–31 μM] | 1.8 |
| HIV RevNES | LQLPPLERLTLDC | 1.8 μM[0.9–1.4 μM]a | 4.1 μM[3.6–4.7 μM] | 2.3 |
| Smad4NES | YERVVSPGIDLSGLTLQ | 4.6 μM[3.6–5.9 μM]a | 13 μM[10–17 μM] | 2.8 |
| FMRP1bNES | LKEVDQLRALERLQID | 3 μM[2.3–3.9 μM]a | 9 μM[5–18 μM] | 3.0 |
| Dusp6NES | PLPVLGLGGLRISS | 4.5 μM[3.7–5.1 μM] | 14 μM[10–18 μM] | 3.1 |
| Cpeb4NES | RTFDMHSLESSIDIMR | 590 nM[400–840 nM]a | 1900 nM[1.5–2.3 μM] | 3.2 |
| IκβεNES | SLVLLPFDDLKISG | 3.6 μM[3.3–4.0 μM] | 12 μM[10–14 μM] | 3.3 |
| PaxillinNES | RELDELMASLSDFKFMA | 670 nM[400–1000 nM]a | 3.1 µM[2.5–3.9 μM] | 4.6 |
| PKINES | NSNELALKLAGLDINK | 35 nM[31–39 nM] | 200 nM[170–30 nM] | 6.3 |
| PP2A B56αNES | REELWKKLEELKLKK | 4.4 μM[3.5–5.4 μM] | 38 μM[28–55 μM] | 8.6 |
| eIF4E-transporter (4E-TNES) | SVEEVEAGLKGLKVDQQVK | 1.4 μM[1.2–1.7 μM] | 14 μM[11–18 μM] | 10 |
68.3% confidence intervals are reported in brackets.
aKds measured previously (Fung et al., 2015, 2017; Fu et al., 2018).