TABLE 1:
Strains and plasmids used in this study.
| Strain/plasmid | Genotype/description | Source |
|---|---|---|
| LWY6474 | MATa, leu2, 3-112, ura3-52, his3-Δ200, trp1-Δ901, lys2-801, suc2-Δ9, fig4Δ::TRP1 | Duex et al., 2006b |
| LWY6538 | MATα, leu2,3-112, ura3-52, his-Δ200, trp1-Δ901, lys2-801, suc2-Δ9, fig4Δ::TRP1, vac14Δ::TRP1 | Duex et al., 2006b |
| LWY8640 | MATa, leu2,3-112, ura3-52, his-Δ200, trp1-Δ901, lys2-801, suc2-Δ9, fig4Δ::TRP1, VAC14-Venus::KAN | Jin et al., 2008 |
| LWY8812 | MATa, leu2,3-112, ura3-52, his-Δ200, trp1-Δ901, lys2-801, suc2-Δ9, fig4Δ::TRP1, FAB1-3XGFP::KAN | Jin et al., 2008 |
| LWY14459 | MATa, leu2,3-112, ura3-52, his-Δ200, trp1-Δ901, lys2-801, suc2-Δ9, fig4Δ::TRP1, VAC14-Venus::KAN, FAB1-TAP::KAN | This study |
| LWY15782 | MATa, leu2,3-112, ura3-52, his-Δ200, trp1-Δ901, lys2-801, suc2-Δ9, fig4Δ::TRP1, VAC14-Venus::KAN, FAB1-6xHA::NAT | This study |
| pRS415-Fig4 | Yeast centromere plasmid; LEU2 | Jin et al., 2008 |
| pRS415-Fig4-C467S | Yeast centromere plasmid; LEU2 | This study |
| pRS415-Fig4-Y538A | Yeast centromere plasmid; LEU2 | This study |
| pRS415-Fig4-E72K | Yeast centromere plasmid; LEU2 | This study |
| pRS415-Fig4-E51K | Yeast centromere plasmid; LEU2 | This study |
| pRS415-Fig4-E51KT52AE72K | Yeast centromere plasmid; LEU2 | This study |
| pRS415-Fig4-T52A | Yeast centromere plasmid; LEU2 | This study |
| pRS415-Fig4-T62A | Yeast centromere plasmid; LEU2 | This study |
| pRS415-Fig4-T78A | Yeast centromere plasmid; LEU2 | This study |
| pRS415-Fig4-T52AT62A | Yeast centromere plasmid; LEU2 | This study |
| pRS415-Fig4-T52AT62AT78A | Yeast centromere plasmid; LEU2 | This study |
| pRS415-Fig4-T52E | Yeast centromere plasmid; LEU2 | This study |
| pRS415-Fig4-T52ET62ET78E | Yeast centromere plasmid; LEU2 | This study |
| pRS415-Fig4-4xMyc | Yeast centromere plasmid; LEU2 | Jin et al., 2008 |
| pRS415-Fig4-4xMyc-C467S | Yeast centromere plasmid; LEU2 | This study |
| pRS415-Fig4-4xMyc-EEE | Yeast centromere plasmid; LEU2 | This study |
| pRS415-Fig4-4xMyc-AAA | Yeast centromere plasmid; LEU2 | This study |
| pRS415ADH | Yeast centromere plasmid; LEU2 | Mumberg et al., 1995 |
| pRS415ADH-Fig4-CTerm | Yeast centromere plasmid; LEU2 | This study |
| pRS413ADH | Yeast centromere plasmid; HIS3 | Mumberg et al., 1995 |
| pRS413ADH-Fig4-NTerm | Yeast centromere plasmid; HIS3 | This study |
| pRS413ADH-Fig4-NTerm-C467S | Yeast centromere plasmid; HIS3 | This study |
| pRS413ADH-Fig4-NTerm-EEE | Yeast centromere plasmid; HIS3 | This study |
| pRS413ADH-Fig4-NTerm-AAA | Yeast centromere plasmid; HIS3 | This study |
| pRS413-Fig4-Envy | Yeast centromere plasmid; HIS3 | This study |
| pRS413-Fig4-Envy-C467S | Yeast centromere plasmid; HIS3 | This study |
| pRS413-Fig4-Envy-Y538A | Yeast centromere plasmid; HIS3 | This study |
| pRS416 Fab1-3xGFP | Yeast centromere plasmid; URA3 | Jin et al., 2008 |