Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Aug 1;31(17):1892–1903. doi: 10.1091/mbc.E19-10-0569

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 Zhang et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License.

PMC Copyright notice

FIGURE 2: — Cdk1 phosphorylation in Ska3 is essential for Ska localization at kinetochores. (A) Thymidine-arrested HeLa Tet-On cells expressing GFP-Ska3 WT, 2A, 4A1/2, 6A, or 6D were released into fresh medium. Mitotic cells were collected for immunostaining using the indicated antibodies. Representative images were shown here. Scale bars, 5 μm. (B) Quantification of in GFP-Ska3 intensity on kinetochores in A. Detailed description about quantification was recorded in Materials and Methods section. At least 60 kinetochores (6 kinetochores per cell) were analyzed for each condition. Average and SD were shown in lines. ****P < 0.0001; n.s. denotes no significance. (C) Cells expressing GFP-Ska3 WT, 4A3, or 6D were treated similarly to the ones in A. Scale bars, 5 μm. (D) Quantification of GFP-Ska3 intensity on kinetochores in C. At least 60 kinetochores (6 kinetochores per cell) were analyzed for each condition. Average and SD were shown in lines; n.s. denotes no significance. (E, G) Live-cell imaging of HeLa Tet-On cells expressing GFP-Ska3 WT, 2A (E), 6A (E), 4A3 (G), or 6D (G) treated with MG132 for 1 h. Scale bars, 5 μm. (F, H) Quantification of GFP-Ska3 intensity on kinetochores (left panel) and microtubules (right panel) in E and G were normalized to that of cytoplasm. Detailed description about quantification was recorded in Materials and Methods section. For both F and H, at least 10 cells (10 kinetochores and 10 microtubules per cell) were analyzed for each condition. Average and SD were shown in lines. *P < 0.05; ****P < 0.0001; n.s. denotes no significance.