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. 2020 Aug 1;31(17):1892–1903. doi: 10.1091/mbc.E19-10-0569

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© 2020 Zhang et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

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FIGURE 6: — A stable macro-complex is formed by the Ska 6D and Ndc80-Nuf2 complexes in vitro. (A–C) The Ska 6D, not WT complex, forms a stable macro-complex with the full-length Ndc80C, not Bonsai. Size-exclusion chromatographic analysis on the mixer of the Ska1-Ska2-Ska3 6D and Nuf2-Ndc80 complexes (A), or the one of the Ska1-Ska2-Ska3 WT and Nuf2-Ndc80 complexes (B), or the one of the Ska1-Ska2-Ska3 6D and the Ndc80 Bosai (C). The thicker lines marked the eluted fractions that were analyzed by SDS–PAGE. The arrows indicate the peaks of the analyzed complexes.