Fig. 3.

IF labeling (red or green stain) of isolated brain MV for markers of endothelial cells, pericytes, astrocytes, and components of BM ECM. MV were isolated from their respective regions of the cerebral cortex. Representative images show MV segments with endothelial cells positive for PECAM (a), vWF (b), PDGFRB (c), GFAP (d). MV segments also retain the BM components laminin (e) and col IV (f). Cell nuclei are labeled with DAPI (blue). Scale bars in a–f are 50 µm