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. 2020 Sep 29;20:473. doi: 10.1186/s12935-020-01549-5

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — MiR-324-3p inhibitor overturned the effects of MALAT1 silencing on HCT116/OxR and HCT8/OxR cells. a The predictive binding sites between MALAT1 and miR-324-3p. b, c Dual-luciferase reporter analysis for the luciferase activities of WT-MALAT1 and MUT-MALAT1 in Ox-resistant CRC cells. d The expression of miR-324-3p in Ox-resistant CRC tissues (n = 40) and Ox-sensitive CRC tissues (n = 40). e The relationship between MALAT1 and miR-324-3p in Ox-resistant CRC tissues (n = 40). f The level of miR-324-3p in Ox-resistant CRC cells, CRC cells and FHT cells. g The transfection efficiency of anti-miR-324-3p in Ox-resistant cells. h–p HCT116/OxR and HCT8/OxR cells were transfected with sh-NC, sh-MALAT1, sh-MALAT1 + anti-miR-NC, or sh-MALAT1 + anti-miR-324-3p. h, i The measurement of IC50 value in HCT116/OxR and HCT8/OxR cells response to Ox. j, k Detection of cell viability by CCK-8 assay. l, m Measurement of cell migration using transwell assay. n Detection of cell apoptosis by flow cytometry. o, p The expression of Bcl-2, Cleaved caspase-3, E-cadherin and Vimentin was determined by western blot. N = 3, *P < 0.05