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. 2020 Sep 29;20:9. doi: 10.1186/s12895-020-00106-x

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Species identification based on conventional mycological tests. a-c. Appearance of Malassezia species on MLNA medium after five days incubation at 32 °C, a. Malassezia furfur, b. Malassezia restricta, and c. Malassezia sympodialis; (d) differences in the ability to grow at 40 °C between isolated strains, I. Malassezia furfur, II. Malassezia sympodialis, and III. Malassezia restricta; (e-g) appearance of Malassezia species on CHROMagar Malassezia medium after four days of incubation at 32 °C, e. Malassezia furfur, f. Malassezia restricta, and g. Malassezia sympodialis; (h-j) micromorphology, typical for each species, observed with DIC microscopy, h. Malassezia furfur, i. Malassezia restricta, and j. Malassezia sympodialis, magnification: (h-j) 1000x; (k-m) differences in catalase activity between three isolated Malassezia strains. k. M. furfur, l. M. restricta, and M. M. sympodialis; (n, o) differences in β-glucosidase activity after 24 h (n) and 120 h (o) between three isolated Malassezia strains, I. M. sympodialis, II. M. restricta, and III. M. furfur. Scale bars correspond to: 10 mm in case of a, b, c, e, f, g and 10 μm in case of h, i, j