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Optimum time duration of G. truncatula LAMP assay. This was determined by undertaking a LAMP assay (a) and confirmation of results of the LAMP assay products using agarose gel (2%) electrophoresis (b). Time durations ranged from 10–40 min and 10-fold dilutions ranged from 104–106 of 34.9 ng/μl G. truncatula genomic DNA extract sample concentration. Lane M: 100 bp DNA Ladder (Thermo Fisher Scientific); Lane +: G. truncatula positive control; Lane−: no-template negative control
