Table 2.

Comparison of G. truncatula LAMP and PCR assays using environmental water samples

Date collected	Habitat	LAMP (n/N)	PCR (n/N)
Farm A
03/05/2019	1	2/2	0/2
03/05/2019	2	0/2	0/2
09/05/2019	1	1/2	1/2
09/05/2019	2	1/2	1/2
16/05/2019	1	1/2	1/2
16/05/2019	2	2/2	2/2
30/05/2019	1	2/2	2/2
30/05/2019	2	2/2	2/2
06/06/2019	1	2/2	2/2
06/06/2019	2	1/2	1/2
13/06/2019	1	2/2	2/2
13/06/2019	2	1/2	2/2
Farm B
03/05/2019	3	1/2	0/2
03/05/2019	4	0/2	0/2
09/05/2019	3	0/2	1/2
09/05/2019	4	0/2	1/2
16/05/2019	3	1/2	2/2
16/05/2019	4	1/2	1/2
30/05/2019	3	1/2	1/2
30/05/2019	4	2/2	1/2
06/06/2019	3	1/2	0/2
06/06/2019	4	0/2	1/2
13/06/2019	3	2/2	2/2
13/06/2019	4	1/2	1/2

Notes: eDNA samples were collected in four locations at Farm A and Farm B over a 7-week period. Area characteristics of the habitats were: 1, stream; 2, water pools; 3, stream; 4, ditch (as shown in Fig. 1)

Abbreviation: n/N, positive/examined