Fig. 1.

Let-7b-5p enhances CD206 expression in macrophages. a Let-7b-5p expression in different macrophages. Human monocytes were isolated from normal donor blood using anti-CD14 magnetic beads, and M0, M1, and M-CMs were differentiated as described in “Materials and methods”. M-CMs were transfected with let-7b-5p inhibitors or NC, or remained untreated for 72 h; M1 macrophages were transfected with let-7b-5p mimics. Relative expression of let-7b-5p was analyzed with qPCR. The expression of miRNA was normalized to U6. Data represent the mean ± standard deviation (SD) of 3 independent experiments. *P < 0.05; **P < 0.01. b CD206 expression in macrophages. After M1 and M-CMs were transfected with let-7b-5p mimics or inhibitors, respectively, CD206 expression of macrophage subtypes was detected with anti-CD-206 PE (red) by the High Throughput Connotation of Imaging System (original magnification, 100×)