Fig. 3.

SOCS1 is a direct target gene of let-7b-5p in vitro. a Protein expression of SOCS1 in macrophages of each group. Expression of SOCS1 protein was confirmed by ELISA after M1 or M-CMs were transfected with let-7b-5p mimics or inhibitors, respectively, for 72 h, compared with the respective controls. b Illustration of the sequence match between let-7b-5p and SOCS1 3′-UTR as determined using TargetScan. c Relative luciferase activity was assayed and calculated by the ratio of firefly-Renilla luciferase activity following transfection with let-7b-5p mimic compared with transfection with NC in 293 T. The data are shown as the mean ± SD of 3 separate experiments. *P < 0.05; **P < 0.01