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. 2020 Sep 29;20:470. doi: 10.1186/s12935-020-01563-7

Fig. 4.

Fig. 4

Overexpression or knockdown of let-7b-5p influences the activity of the STAT-signaling pathway. Macrophage cells of each group were harvested and lysed, and the supernatant was collected. After the protein concentration was determined using the Bio-Rad protein assay, 40 µg of cell lysates from each group was used to analyze the expression of the signaling proteins of macrophages using the CST signal-pathway chip according to the manufacturer’s instructions. These data were visualized with the Bio-Rad imager