Fig. 5.

Let-7b-5p reduces phagocytosis by macrophages. After FITC-Staphylococcus aureus antigen was added to normal, cultured macrophages in each group at a ratio of 1:20 (S. aureus:macrophages), the mixture was shielded from light and incubated at 37 °C for 20 min. The supernatant was removed, and we recorded phagocytosis by macrophages in each group and quantified them by measuring the numbers of macrophages with FITC-S. aureus antigen in a field using the High Throughput Connotation of Imaging System (original magnification, 100×). *P < 0.05; **P < 0.01; ***P < 0.001