Table 2.
Expected and observed results of PCR and LAMP diagnostic marker for MS1 in Cryptomeria japonica
| Maker ID | Expected size (bp)#1 or presence/absence (±) of products | Observed size (bp) or presence/absence (±) of products | ||||
|---|---|---|---|---|---|---|
| Ms1 (wild allele) | ms1-1 (4-bp deletion) | ms1-2 (30-bp deletion) | Ms1 (wild allele) | ms1-1 (4-bp deletion) | ms1-2 (30-bp deletion) | |
| ALP_ms1-1 | 165 | 161 | 165 | 162 | 158 | 162 |
| ALP_ms1-2 | 146 | 146 | 116 | 141 | 141 | 112 |
| ALP_ms1 | 172/288#2 | 168 | 258 | 169/286#2 | 165 | 255 |
| ASP_ms1-1 | – | 103 | – | – | + | – |
| ASP_ms1-1_wt | 105 | – | 105 | + | – | + |
| LAMP_ms1-1 | – | + | – | – | + | – |
| LAMP_ms1-1_wt | + | – | + | + | – | + |
| LAMP_ms1-2 | – | – | + | +#3 | +#3 | + |
#1Tail sequences of primers are included to calculate the expected PCR product size
#2Wild type allele is expected to produce fragments with 172 and 288 bp without 4- and 30-bp deletions, respectively
#3Products are still absent after 60 min incubation, but present after 120 min incubation (Additional file 6)