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. 2020 Sep 29;6:68. doi: 10.1038/s41421-020-00210-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a The overall complex structure of cACE2 bound to SARS-CoV-2 RBD. cACE2 and SARS-CoV-2 RBD were colored in lightpink and palecyan, respectively. b, c The detailed interaction between cACE2 and the SARS-CoV-2 RBD. The residues involved in the interaction were labeled, and H-bonds were shown as dotted lines with a cutoff of 3.3 Å. d The overall comparison between the complex of cACE2 and SARS-CoV-2 RBD and that of hACE2 and SARS-CoV-2 RBD. hACE2 and SARS-CoV-2 RBD complex were colored in gray. SARS-CoV-2 RBDs and ACE2s were displayed in ribbon and cartoon, respectively. Residue D355 of ACE2 and interacted residues on SARS-CoV-2 RBD were circled, related to Supplementary Fig. S2c, d. e HEK293T cells transfected with pEGFP-N1-cACE2, hACE2 (WT), or the mutants containing D355A were incubated with His-tagged SARS-CoV-2 RBD protein. Anti-His/APC antibody was used to detect the His-tagged protein binding to the cells by flow cytometry. The percentage of the indicated ACE2-expressing cells that were bound to SARS-CoV-2 RBD were shown as a histogram. The assays were independently performed twice. One representative data was displayed with the mean of triplicates (n = 3), and the bar represented the SD value.