Figure 7. Expression of TRPV4 channel in native rat choroid plexus.

(A) Immunoblotting of WT choroid plexus (WT CPe) and hydrocephalic choroid plexus (Hom CPe) for TRPV4 protein using β-actin and Ponceau S loading controls, showing no substantial change in protein expression of TRPV4 due to genotype. (B) Immunoblotting of WT kidney (WT Kid), hydrocephalic kidney (Hom Kid), WT choroid plexus (WT CPe), and hydrocephalic choroid plexus (Hom CPe) for TRPV4. (C) Deglycosylation of TRPV4 with PNGaseF enzyme with matched untreated inputs demonstrating that there are 2 isoforms of TRPV4, both of which are glycosylated in the choroid plexus. There is only 1 isoform of TRPV4 in the kidney, but it is also glycosylated. Significance values were determined by paired t test in Prism between experimental groups, and no significant differences were found.