Figure 5. Abnormal mitochondria structure in the Dnm2^SL/+ mice.

(A) Subcellular localization of DNM2 by immunofluorescence in longitudinal sections from TA muscles at 8w. Scale bar: 20 μm. (B) Ultrastructural analysis by electron microscopy in WT and Dnm2^SL/+ TA muscles at 8w (n = 2 replicates per group). Arrows point to Z-line misalignment, and stars indicate swollen mitochondria. Scale bar: 2 μm. (C and D) Higher magnification showing swollen mitochondria with disrupted cristae in the Dnm2^SL/+ muscle only. Scale bars: 0.5 μm (C) and 500 nm (D). (E) Electron microscopy (Scale bar: 2 μm) and H&E (100× magnification) representative images from a muscle from a patient carrying the S619L mutation.