Figure 7. CNM histological hallmarks of Dnm2^SL/+ mice are ameliorated upon DNM2 reduction.

(A) H&E, SDH, and NADH stainings of TA muscle sections from 8-week-old WT and Dnm2^SL/+ mice treated or not with ASO-1. Scale bar: 50 μm (n = 5 per group and staining). Inset, 2× magnification. (B–D) Quantification of fiber size (n = 4, graph shows average value ± SEM for each diameter and genotype) (B), nuclei internalization and centralization (n = 4) (C), and percentage of fibers with central accumulation of SDH stain (n = 4) (D). (E) DNM2 protein level in TA muscles of 8-week-old WT and Dnm2^SL/+ mice treated or not with ASO-1 (4 ≤ n ≤ 5; 1-way ANOVA with Tukey’s post hoc test). ****P < 0.0001 and **P < 0.01 comparing vs. WT. ^$$$$P < 0.0001 vs. Dnm2^SL/+. Charts show individual points with additional lines indicating mean ± SEM unless differently stated.