Figure 9. uTregs show site-specific adaptation to the materno-fetal interface.

(A) Heatmap with previously highlighted genes in this manuscript that were differentially expressed between ^pbuTregs and ^incuTregs. Expression values were mean centered and scaled per gene. (B and C) Protein expression of PD-1 (B) and OX-40 (C). uTregs were gated as CD3⁺CD4⁺CD25^hiFOXP3⁺ cells. Multiplicity adjusted P value of 2-way ANOVA with Tukey’s post hoc test. Left upper P value, blood versus placental bed; right upper P value, placental bed versus incision site; lower P value, blood versus incision site. MFI, median fluorescence intensity (n = 6). (D) GSEA with published TITR-specific signatures in ^pbuTregs versus ^incuTregs (25, 95–100). NES, normalized enrichment score; FDR, FDR adjusted P value. (E) GSEA with published gene signatures specific to Tregs from tumor tissue compared with the healthy tissue counterpart in ^pbuTregs versus ^incuTregs (26, 100). (F) Protein expression of CCR8 (n = 5), ICOS (n = 6), and GITR (n = 6). uTregs were gated as CD3⁺CD4⁺CD25^hiFOXP3⁺ cells. Multiplicity adjusted P value of 2-way ANOVA with Tukey’s post hoc test for protein. Left upper P value, blood versus placental bed; right upper P value, placental bed versus incision site; lower P value, blood versus incision site. MFI, median fluorescence intensity. ****P < 0.0001, ***P < 0.001, **P < 0.01, *P < 0.05.