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. 2020 Sep 17;5(18):e139640. doi: 10.1172/jci.insight.139640

Figure 2. siRNA silencing of HSPA12B or YAP attenuates hypoxia-induced endothelial cell proliferation, migration, and angiogenesis.

Figure 2

HUVECs were transfected with siRNA specific for HSPA12B (siHSPA12B) or for YAP (siYAP). Scrambled siRNA served as control (siNC). Twenty-four hours after transfection, the cells were subjected to hypoxia or normoxia. Cell proliferation was measured by Edu incorporation (n = 3) (A) and MTT assay (n = 4) (B) (scale bar: 400 μm). (C) Cell migration was examined by wound-healing assay (n = 3) (scale bar: 1000 μm). (D) The levels of Ang1 (n = 3), VEGF (n = 4), and VEGFR2 (n = 3) were examined by Western blot. GAPDH was used as loading control. Comparisons of data between groups were made using 1-way ANOVA followed by Tukey’s procedure. *P < 0.05, **P < 0.01, ***P < 0.001 compared with indicated groups. HUVECs, human umbilical vein endothelial cells; YAP, yes-associated protein; Edu, 5-ethynyl-2-deoxyuridine.