Table 1.
Primer sequences for the LAMP assays specific for Dracunculus medinensis, D. insignis and the copepod internal positive control.
| Primer | Sequence (5′–3′) |
|---|---|
| D.med F3 | GTTATTACTTCTCATGCTATTATGATA |
| D.med B3 | AAACTAAAAATAGCCAAATCAACTCTAT |
| D.med FIP | ACAGGCATCAATCAATAACTAACATTATTCTGGTAATGCCTAGTTTGATTGGA |
| D.med BIP | AATATTGATTTTGTCTGCTTGTTTGGTGCCAGGATGACCCCTAGTACTT |
| D.med F-loop | ATATCAGGAGCCCCCAACATT |
| D.med B-loop |
TCTTGTGGAACTAGATGGACTA |
| D.insig F3 | TTTTTATGGTTATGCCTAGTTTGATT |
| D.insig B3 | CAGAACAATGTAAACTAAAAATAGCCA |
| D.insig FIP | CATCAAAGAAACAGGTATTAACCAATAACGGTGGTTTTGGTAATTGGATAGTT |
| D.insig BIP | AGTCGATAGTTCTTGTGGTACTAGTTCAACACTATTTCCAGGATGACCA |
| D.insig F-loop | CACATTATTCAAACGAGGAAAACTT |
| D.insig B-loop |
GAACTGTTTATCCTCCTTTGAGT |
| Copepod F3 | GTCGACTGTGGCATAGACG |
| Copepod B3 | CTCATTCCGATTACCAGGCC |
| Copepod FIP | TCAGGCTCCCTCTCCGGAATCCCACAGTGGTTTTGACGG |
| Copepod BIP | AAGGCAGCAGGCACGCAAATTCGGATGAGTCTGGTATCGT |
| Copepod F-loop | CGAACCCTAATTCCCCGTTAC |
| Copepod B-loop | GCCGAGGTAGTGACGAAAAAT |