Table 3.
Summary of real-time LAMP results testing samples comprising Dracunculus-free copepods spiked with D. medinensis or D. insignis larvae. Nt = sample not tested, - = negative result. -ve sample is a no-template control, +ve controls are copepods mixed with Dracunculus DNA. Tp = time to positive, Ta = Annealing temperature.
| Number spiked into sample |
Samples tested with LAMP assays for |
|||||||
|---|---|---|---|---|---|---|---|---|
| Copepods | D. medinensis | D. insignis |
D. medinensis |
D. insignis |
Copepod |
|||
| Tp (min:sec) | Ta (°C) | Tp (min:sec) | Ta (°C) | Tp (min:sec) | Ta (°C) | |||
| 1 | 1 | 0 | 8:15 | 82.20 | nt | nt | 6:30 | 87.49 |
| 10 | 1 | 0 | 7:45 | 82.07 | nt | nt | 6:15 | 87.65 |
| 20 | 1 | 0 | 8:00 | 82.09 | nt | nt | 5:15 | 87.78 |
| 30 | 1 | 0 | 8:15 | 82.04 | nt | nt | 5:30 | 87.79 |
| 30 |
1 |
0 |
8:45 |
81.97 |
nt |
nt |
5:30 |
87.76 |
| 10 | 0 | 0 | – | – | nt | nt | 6:30 | 87.21 |
| 20 | 0 | 0 | – | – | nt | nt | 6:00 | 87.75 |
| ≥50 | 0 | 0 | – | – | nt | nt | 6:15 | 87.16 |
| ≥100 |
0 |
0 |
– |
– |
nt |
nt |
6:15 |
87.21 |
| 1 | 0 | 1 | nt | nt | 10:15 | 82.50 | 9:00 | 88.26 |
| 10 | 0 | 1 | nt | nt | 13:00 | 82.49 | 7:30 | 88.31 |
| 10 |
0 |
0 |
nt |
nt |
– |
– |
7:00 |
88.26 |
| -ve control | – | – | – | – | – | – | ||
| +ve control | 7:30 | 81.83 | nt | nt | 6:00 | 87.67 | ||
| +ve control | nt | nt | 11:30 | 82.42 | 8:00 | 88.41 | ||