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. Author manuscript; available in PMC: 2020 Nov 6.
Published in final edited form as: Nat Struct Mol Biol. 2019 Nov 6;26(11):1023–1034. doi: 10.1038/s41594-019-0320-0

Table 1 |.

CryoEM data collection, refinement and validation statistics (asymmetric reconstruction)

q-CPV (EMD-20595) S-CPV (EMD-20596) SG-CPV (EMD-20597) SA-CPV (EMD-20598) SGA-CPV (EMD-20599) SGAU-CPV (EMD-20600) t-CPV
(EMD-20601)
Data collection and processing
 Magnification 130,000 130,000 130,000 130,000 130,000 130,000 105,000
 Voltage (kV) 300 300 300 300 300 300 300
 Electron exposure (e per Å2) ~44 ~40 ~48 ~50 ~44 ~48 ~40
 Defocus range (μm) 0.8–3 0.8–3 0.8–3 0.8–3 0.8–3 0.8–3 0.8–3
 Pixel size (Å) 1.062 1.062 1.062 1.062 1.062 1.062 1.360
 Symmetry imposed C1 C1 C1 C1 C1 C1 C1
 Initial particle images (no.) 95,428 22,795 42,429 33,093 191,055 56,227 89,587
 Final particle images (no.) 82,420 22,465 41,969 28,854 182,525 55,129 89,587
 Map resolution (Å) 3.7 3.9 3.7 4.0 3.1 3.6 4.1
 FSC threshold 0.143 0.143 0.143 0.143 0.143 0.143 0.143
 Map resolution range (Å) 3–30 3.1–30 3–30 3.5–30 2.5–30 2.9–30 3.5–30