Table 1 |.
q-CPV (EMD-20595) | S-CPV (EMD-20596) | SG-CPV (EMD-20597) | SA-CPV (EMD-20598) | SGA-CPV (EMD-20599) | SGAU-CPV (EMD-20600) | t-CPV (EMD-20601) |
|
---|---|---|---|---|---|---|---|
Data collection and processing | |||||||
Magnification | 130,000 | 130,000 | 130,000 | 130,000 | 130,000 | 130,000 | 105,000 |
Voltage (kV) | 300 | 300 | 300 | 300 | 300 | 300 | 300 |
Electron exposure (e− per Å2) | ~44 | ~40 | ~48 | ~50 | ~44 | ~48 | ~40 |
Defocus range (μm) | 0.8–3 | 0.8–3 | 0.8–3 | 0.8–3 | 0.8–3 | 0.8–3 | 0.8–3 |
Pixel size (Å) | 1.062 | 1.062 | 1.062 | 1.062 | 1.062 | 1.062 | 1.360 |
Symmetry imposed | C1 | C1 | C1 | C1 | C1 | C1 | C1 |
Initial particle images (no.) | 95,428 | 22,795 | 42,429 | 33,093 | 191,055 | 56,227 | 89,587 |
Final particle images (no.) | 82,420 | 22,465 | 41,969 | 28,854 | 182,525 | 55,129 | 89,587 |
Map resolution (Å) | 3.7 | 3.9 | 3.7 | 4.0 | 3.1 | 3.6 | 4.1 |
FSC threshold | 0.143 | 0.143 | 0.143 | 0.143 | 0.143 | 0.143 | 0.143 |
Map resolution range (Å) | 3–30 | 3.1–30 | 3–30 | 3.5–30 | 2.5–30 | 2.9–30 | 3.5–30 |